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Showing Protein Collagenase ColG (HMDBP14151)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 0 metabolites
Identification
HMDB Protein ID HMDBP14151
Secondary Accession Numbers None
Name Collagenase ColG
Synonyms
  1. Class I collagenase
  2. Gelatinase ColG
  3. Microbial collagenase
Gene Name COLG
Protein Type Unknown
Biological Properties
General Function Not Available
Specific Function Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen (PubMed:3002446). Active on soluble type I collagen, insoluble collagen, azocoll, soluble PZ-peptide (all collagenase substrates) and gelatin (PubMed:9922257). The full-length protein has collagenase activity, while the in vivo derived C-terminally truncated shorter versions only act on gelatin (PubMed:9922257). In vitro digestion of soluble calf skin collagen fibrils requires both ColG and ColH; ColG forms missing the second collagen-binding domain are also synergistic with ColH, although their overall efficiency is decreased (PubMed:18374061, PubMed:22099748). The activator domain (residues 119-388) and catalytic subdomain (389-670) open and close around substrate using a Gly-rich hinge (387-397), allowing digestion when the protein is closed (PubMed:21947205, PubMed:23703618). Binding of collagen requires Ca(2+) and is inhibited by EGTA; the collagen-binding domain (CBD, S3a plus S3b) specifically recognizes the triple-helical conformation made by 3 collagen protein chains in the triple-helical region (PubMed:11121400). Isolated CBD (S3a plus S3b) binds collagen fibrils and sheets of many tissues (PubMed:11913772).
Pathways Not Available
Reactions Not Available
GO Classification
Biological Process
pathogenesis
collagen metabolic process
Cellular Component
extracellular region
Molecular Function
zinc ion binding
calcium ion binding
endopeptidase activity
collagen binding
metalloendopeptidase activity
serine-type endopeptidase activity
tripeptidase activity
Cellular Location Not Available
Gene Properties
Chromosome Location Not Available
Locus Not Available
SNPs Not Available
Gene Sequence Not Available
Protein Properties
Number of Residues Not Available
Molecular Weight 126240.855
Theoretical pI Not Available
Pfam Domain Function
Signals
  • 1-45;
Transmembrane Regions Not Available
Protein Sequence Not Available
GenBank ID Protein Not Available
UniProtKB/Swiss-Prot ID Q9X721
UniProtKB/Swiss-Prot Entry Name COLG_HATHI
PDB IDs
GenBank Gene ID Not Available
GeneCard ID Not Available
GenAtlas ID Not Available
HGNC ID Not Available
References
General References
  1. Matsushita O, Jung CM, Katayama S, Minami J, Takahashi Y, Okabe A: Gene duplication and multiplicity of collagenases in Clostridium histolyticum. J Bacteriol. 1999 Feb;181(3):923-33. doi: 10.1128/JB.181.3.923-933.1999. [PubMed:9922257 ]
  2. Bond MD, Van Wart HE: Characterization of the individual collagenases from Clostridium histolyticum. Biochemistry. 1984 Jun 19;23(13):3085-91. doi: 10.1021/bi00308a036. [PubMed:6087888 ]
  3. Mookhtiar KA, Steinbrink DR, Van Wart HE: Mode of hydrolysis of collagen-like peptides by class I and class II Clostridium histolyticum collagenases: evidence for both endopeptidase and tripeptidylcarboxypeptidase activities. Biochemistry. 1985 Nov 5;24(23):6527-33. doi: 10.1021/bi00344a033. [PubMed:3002446 ]
  4. Fecteau KA, Haffner JC, Eiler H: The potential of collagenase as a new therapy for separation of human retained placenta: hydrolytic potency on human, equine and bovine placentae. Placenta. 1998 Jul-Aug;19(5-6):379-83. doi: 10.1016/s0143-4004(98)90077-7. [PubMed:9699958 ]
  5. Badalamente MA, Hurst LC: Enzyme injection as nonsurgical treatment of Dupuytren's disease. J Hand Surg Am. 2000 Jul;25(4):629-36. doi: 10.1053/jhsu.2000.6918. [PubMed:10913202 ]
  6. Toyoshima T, Matsushita O, Minami J, Nishi N, Okabe A, Itano T: Collagen-binding domain of a Clostridium histolyticum collagenase exhibits a broad substrate spectrum both in vitro and in vivo. Connect Tissue Res. 2001;42(4):281-90. doi: 10.3109/03008200109016842. [PubMed:11913772 ]
  7. Matsushita O, Koide T, Kobayashi R, Nagata K, Okabe A: Substrate recognition by the collagen-binding domain of Clostridium histolyticum class I collagenase. J Biol Chem. 2001 Mar 23;276(12):8761-70. doi: 10.1074/jbc.M003450200. Epub 2000 Dec 19. [PubMed:11121400 ]
  8. McCarthy RC, Spurlin B, Wright MJ, Breite AG, Sturdevant LK, Dwulet CS, Dwulet FE: Development and characterization of a collagen degradation assay to assess purified collagenase used in islet isolation. Transplant Proc. 2008 Mar;40(2):339-42. doi: 10.1016/j.transproceed.2008.01.041. [PubMed:18374061 ]
  9. Eckhard U, Schonauer E, Ducka P, Briza P, Nuss D, Brandstetter H: Biochemical characterization of the catalytic domains of three different Clostridial collagenases. Biol Chem. 2009 Jan;390(1):11-8. doi: 10.1515/BC.2009.004. [PubMed:18937627 ]
  10. Shi L, Carson D: Collagenase Santyl ointment: a selective agent for wound debridement. J Wound Ostomy Continence Nurs. 2009 Nov-Dec;36(6 Suppl):S12-6. doi: 10.1097/WON.0b013e3181bfdd1a. [PubMed:19918145 ]
  11. Hurst LC, Badalamente MA, Hentz VR, Hotchkiss RN, Kaplan FT, Meals RA, Smith TM, Rodzvilla J: Injectable collagenase clostridium histolyticum for Dupuytren's contracture. N Engl J Med. 2009 Sep 3;361(10):968-79. doi: 10.1056/NEJMoa0810866. [PubMed:19726771 ]
  12. Breite AG, McCarthy RC, Dwulet FE: Characterization and functional assessment of Clostridium histolyticum class I (C1) collagenases and the synergistic degradation of native collagen in enzyme mixtures containing class II (C2) collagenase. Transplant Proc. 2011 Nov;43(9):3171-5. doi: 10.1016/j.transproceed.2011.09.059. [PubMed:22099748 ]
  13. Philominathan ST, Koide T, Matsushita O, Sakon J: Bacterial collagen-binding domain targets undertwisted regions of collagen. Protein Sci. 2012 Oct;21(10):1554-65. doi: 10.1002/pro.2145. [PubMed:22898990 ]
  14. Eckhard U, Huesgen PF, Brandstetter H, Overall CM: Proteomic protease specificity profiling of clostridial collagenases reveals their intrinsic nature as dedicated degraders of collagen. J Proteomics. 2014 Apr 4;100:102-14. doi: 10.1016/j.jprot.2013.10.004. Epub 2013 Oct 11. [PubMed:24125730 ]
  15. Schonauer E, Kany AM, Haupenthal J, Husecken K, Hoppe IJ, Voos K, Yahiaoui S, Elsasser B, Ducho C, Brandstetter H, Hartmann RW: Discovery of a Potent Inhibitor Class with High Selectivity toward Clostridial Collagenases. J Am Chem Soc. 2017 Sep 13;139(36):12696-12703. doi: 10.1021/jacs.7b06935. Epub 2017 Aug 31. [PubMed:28820255 ]
  16. Wilson JJ, Matsushita O, Okabe A, Sakon J: A bacterial collagen-binding domain with novel calcium-binding motif controls domain orientation. EMBO J. 2003 Apr 15;22(8):1743-52. doi: 10.1093/emboj/cdg172. [PubMed:12682007 ]
  17. Eckhard U, Brandstetter H: Polycystic kidney disease-like domains of clostridial collagenases and their role in collagen recruitment. Biol Chem. 2011 Nov;392(11):1039-45. doi: 10.1515/BC.2011.099. Epub 2011 Aug 28. [PubMed:21871007 ]
  18. Eckhard U, Schonauer E, Nuss D, Brandstetter H: Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis. Nat Struct Mol Biol. 2011 Sep 25;18(10):1109-14. doi: 10.1038/nsmb.2127. [PubMed:21947205 ]
  19. Bauer R, Wilson JJ, Philominathan ST, Davis D, Matsushita O, Sakon J: Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum. J Bacteriol. 2013 Jan;195(2):318-27. doi: 10.1128/JB.00010-12. Epub 2012 Nov 9. [PubMed:23144249 ]
  20. Bauer R, Janowska K, Taylor K, Jordan B, Gann S, Janowski T, Latimer EC, Matsushita O, Sakon J: Structures of three polycystic kidney disease-like domains from Clostridium histolyticum collagenases ColG and ColH. Acta Crystallogr D Biol Crystallogr. 2015 Mar;71(Pt 3):565-77. doi: 10.1107/S1399004714027722. Epub 2015 Feb 26. [PubMed:25760606 ]