| Identification |
|---|
| HMDB Protein ID
| HMDBP14057 |
| Secondary Accession Numbers
| None |
| Name
| Lipid A 4'-phosphatase |
| Synonyms
|
Not Available
|
| Gene Name
| LPXF |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Removes the 4'-phosphate moiety from lipid IV(A) (a tetraacylated precursor of lipid A) and from pentaacylated lipid A, but not from hexaacylated lipid A (as is found in E.coli). Does not dephosphorylate phosphatidic acid, phosphatidylglycerophosphate, or the 1-phosphate group of lipid A and lipid A precursors. Its expression in E.coli confers resistance to the cationic antimicrobial peptide (CAMP) polymyxin B (PubMed:16467300). Plays a critical role in the ability of the bacteria to avoid the host's innate immune system, especially the bactericidal action of CAMPs, although whether it is CAMP-sensitivity or increased sensitivity to the immune system is not clear (PubMed:17360489). |
| Pathways
|
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| response to antibiotic |
| lipopolysaccharide biosynthetic process |
| pathogenesis |
| lipid A biosynthetic process |
| Cellular Component |
| integral to membrane |
| plasma membrane |
| Molecular Function |
| hydrolase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 222 |
| Molecular Weight
| 25551.315 |
| Theoretical pI
| 9.364 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
- 4-24;59-79;88-108;145-165;170-190;194-214;
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| A0Q4N6 |
| UniProtKB/Swiss-Prot Entry Name
| LPXF_FRATN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Rohmer L, Fong C, Abmayr S, Wasnick M, Larson Freeman TJ, Radey M, Guina T, Svensson K, Hayden HS, Jacobs M, Gallagher LA, Manoil C, Ernst RK, Drees B, Buckley D, Haugen E, Bovee D, Zhou Y, Chang J, Levy R, Lim R, Gillett W, Guenthener D, Kang A, Shaffer SA, Taylor G, Chen J, Gallis B, D'Argenio DA, Forsman M, Olson MV, Goodlett DR, Kaul R, Miller SI, Brittnacher MJ: Comparison of Francisella tularensis genomes reveals evolutionary events associated with the emergence of human pathogenic strains. Genome Biol. 2007;8(6):R102. doi: 10.1186/gb-2007-8-6-r102. [PubMed:17550600 ]
- Wang X, McGrath SC, Cotter RJ, Raetz CR: Expression cloning and periplasmic orientation of the Francisella novicida lipid A 4'-phosphatase LpxF. J Biol Chem. 2006 Apr 7;281(14):9321-30. doi: 10.1074/jbc.M600435200. Epub 2006 Feb 8. [PubMed:16467300 ]
- Schilling B, McLendon MK, Phillips NJ, Apicella MA, Gibson BW: Characterization of lipid A acylation patterns in Francisella tularensis, Francisella novicida, and Francisella philomiragia using multiple-stage mass spectrometry and matrix-assisted laser desorption/ionization on an intermediate vacuum source linear ion trap. Anal Chem. 2007 Feb 1;79(3):1034-42. doi: 10.1021/ac061654e. [PubMed:17263332 ]
- Wang X, Ribeiro AA, Guan Z, Abraham SN, Raetz CR: Attenuated virulence of a Francisella mutant lacking the lipid A 4'-phosphatase. Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):4136-41. doi: 10.1073/pnas.0611606104. Epub 2007 Feb 26. [PubMed:17360489 ]
|
