| Identification |
|---|
| HMDB Protein ID
| HMDBP13898 |
| Secondary Accession Numbers
| None |
| Name
| 6,7-dimethyl-8-ribityllumazine synthase 2 |
| Synonyms
|
- DMRL synthase 2
- LS 2
- Lumazine synthase 2
- BLS
- Type II lumazine synthase
|
| Gene Name
| RIBH2 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. Displays low catalytic activity in comparison with the isozyme RibH1. Is a highly immunogenic protein. Activates dendritic cells (DCs) in vitro, increasing the levels of costimulatory molecules and the secretion of proinflammatory cytokines, and recruits DCs, B cells and CD8+ T cells in vivo, both effects in a TLR4-dependent manner. Induces the cross presentation of covalently attached peptides and generates a strong and long-lasting humoral immune response without adjuvants; TLR4 signaling is necessary for the induction of the cytotoxic response but not for antigen cross presentation. Elicits a TLR4-mediated protective response against B16 melanoma in mice, slowing tumor growth and prolonging mice survival. |
| Pathways
|
- Biosynthesis of cofactors
- Biosynthesis of secondary metabolites
- riboflavin biosynthesis
- Riboflavin metabolism
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| riboflavin biosynthetic process |
| Cellular Component |
| cytoplasm |
| riboflavin synthase complex |
| Molecular Function |
| 6,7-dimethyl-8-ribityllumazine synthase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 158 |
| Molecular Weight
| 17355.785 |
| Theoretical pI
| 7.118 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P61711 |
| UniProtKB/Swiss-Prot Entry Name
| RISB2_BRUAB |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Hemmen F, Weynants V, Scarcez T, Letesson JJ, Saman E: Cloning and sequence analysis of a newly identified Brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes. Clin Diagn Lab Immunol. 1995 May;2(3):263-7. doi: 10.1128/cdli.2.3.263-267.1995. [PubMed:7664168 ]
- Halling SM, Peterson-Burch BD, Bricker BJ, Zuerner RL, Qing Z, Li LL, Kapur V, Alt DP, Olsen SC: Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. J Bacteriol. 2005 Apr;187(8):2715-26. doi: 10.1128/JB.187.8.2715-2726.2005. [PubMed:15805518 ]
|
