| Identification |
|---|
| HMDB Protein ID
| HMDBP13701 |
| Secondary Accession Numbers
| None |
| Name
| Ricin B-like lectin |
| Synonyms
|
- Clitocybe nebularis lectin
- CNL
|
| Gene Name
| Not Available |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Lectin specific for terminal, non-reducing N-acetylgalactosamine (Gal-NAc)-containing carbohydrates including N,N'-diacetyllactosediamine/LDN (GalNAcbeta1-4GlcNAc, LacdiNAc). Specific also for carbohydrates containing N-acetylglucosamine (-GlcNAc) or N-acetyllactosamine (-Galbeta1-4GlcNAc) at the reducing end. Agglutinates human blood group A, AB, B and O erythrocytes with a strong preference for group A. Agglutinates bovine erythrocytes with a very low specificity (PubMed:19100814, PubMed:22298779). Binds carbohydrates bivalently, which is required for its biological activity (PubMed:22298779). Exhibits insecticidal activity against the fruit fly D.melanogaster, mosquito A.aegypti, and amoebozoa A.castellanii. Has anti-nutritional activity against Colorado potato beetle L.decemlineata, and against worm C.elegans (PubMed:21556921, PubMed:21486374). Has antiproliferative activity against human leukemic T-cells (PubMed:19100814). Has an immunostimulatory effect on human antigen-presenting dendritic cells, which are subsequently able to induce efficient T-cell immune responses (PubMed:22044067). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Molecular Function |
| protein homodimerization activity |
| carbohydrate binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 15992.75 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| B2ZRS9 |
| UniProtKB/Swiss-Prot Entry Name
| CNL_CLINE |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Pohleven J, Obermajer N, Sabotic J, Anzlovar S, Sepcic K, Kos J, Kralj B, Strukelj B, Brzin J: Purification, characterization and cloning of a ricin B-like lectin from mushroom Clitocybe nebularis with antiproliferative activity against human leukemic T cells. Biochim Biophys Acta. 2009 Mar;1790(3):173-81. doi: 10.1016/j.bbagen.2008.11.006. Epub 2008 Dec 6. [PubMed:19100814 ]
- Pohleven J, Renko M, Magister S, Smith DF, Kunzler M, Strukelj B, Turk D, Kos J, Sabotic J: Bivalent carbohydrate binding is required for biological activity of Clitocybe nebularis lectin (CNL), the N,N'-diacetyllactosediamine (GalNAcbeta1-4GlcNAc, LacdiNAc)-specific lectin from basidiomycete C. nebularis. J Biol Chem. 2012 Mar 23;287(13):10602-10612. doi: 10.1074/jbc.M111.317263. Epub 2012 Feb 1. [PubMed:22298779 ]
- Pohleven J, Brzin J, Vrabec L, Leonardi A, Cokl A, Strukelj B, Kos J, Sabotic J: Basidiomycete Clitocybe nebularis is rich in lectins with insecticidal activities. Appl Microbiol Biotechnol. 2011 Aug;91(4):1141-8. doi: 10.1007/s00253-011-3236-0. Epub 2011 May 10. [PubMed:21556921 ]
- Bleuler-Martinez S, Butschi A, Garbani M, Walti MA, Wohlschlager T, Potthoff E, Sabotic J, Pohleven J, Luthy P, Hengartner MO, Aebi M, Kunzler M: A lectin-mediated resistance of higher fungi against predators and parasites. Mol Ecol. 2011 Jul;20(14):3056-70. doi: 10.1111/j.1365-294X.2011.05093.x. Epub 2011 Apr 12. [PubMed:21486374 ]
|
