| Identification |
|---|
| HMDB Protein ID
| HMDBP13506 |
| Secondary Accession Numbers
| None |
| Name
| Thrombin-like enzyme BpirSP27 |
| Synonyms
|
- SVTLE
- Fibrinogen-clotting enzyme
- Snake venom serine protease
- SVSP
|
| Gene Name
| Not Available |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Snake venom serine protease that interferes with the hemostatic system of the prey. It preferentially degrades the Bbeta chain (FGB) of fibrinogen, with minor effects on the Aalpha chain (FGA). It presents a lower ability to degrade fibrin clots than BpirSP41. It hydrolyzes chromogenic substrates S-2238 (used for testing thrombin activity), S-2222 (factor Xa), S-2266 (glandular kallikrein and factor XIa), S-2302 (plasma kallikrein, factor XIa and XIIa), and S-2251 (plasmin). It shows a decrease in the clotting time of human plasma in the presence of increasing doses of the enzyme. Its minimum coagulant dose (MCD) is 3.5 ug. It also promotes platelet aggregation in a concentration-dependent manner in the presence or absence of calcium. It also shows 20% inhibition of the hemolytic activity promoted by the complement pathways and possess only a minor role in the induction of edema and pain in rat. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Cellular Component |
| extracellular region |
| Molecular Function |
| toxin activity |
| serine-type endopeptidase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 5534.175 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P0DL26 |
| UniProtKB/Swiss-Prot Entry Name
| VSP27_BOTPI |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Menaldo DL, Bernardes CP, Santos-Filho NA, Moura Lde A, Fuly AL, Arantes EC, Sampaio SV: Biochemical characterization and comparative analysis of two distinct serine proteases from Bothrops pirajai snake venom. Biochimie. 2012 Dec;94(12):2545-58. doi: 10.1016/j.biochi.2012.07.007. Epub 2012 Jul 17. [PubMed:22819993 ]
- Menaldo DL, Bernardes CP, Pereira JC, Silveira DS, Mamede CC, Stanziola L, Oliveira Fd, Pereira-Crott LS, Faccioli LH, Sampaio SV: Effects of two serine proteases from Bothrops pirajai snake venom on the complement system and the inflammatory response. Int Immunopharmacol. 2013 Apr;15(4):764-71. doi: 10.1016/j.intimp.2013.02.023. Epub 2013 Mar 15. [PubMed:23499645 ]
|
