| Identification |
|---|
| HMDB Protein ID
| HMDBP13500 |
| Secondary Accession Numbers
| None |
| Name
| Thrombin-like enzyme flavoxobin |
| Synonyms
|
- SVTLE
- Fibrinogen-clotting enzyme
- Habutobin
- Snake venom serine protease 1
- SVSP
|
| Gene Name
| TLF1 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Thrombin-like snake venom serine protease that clots fibrinogen (FGA) by releasing fibrinopeptide A. According to PubMed:8585090, only cleaves rabbit fibrinogen, whereas no specificity is described in PubMed:3910643 (tests done on bovine fibrinogen). Also acts as a C3 convertase that independently cleaves human C3 and kick-starts the complement cascade. Also increases urokinase-type plasminogen activator (PLAU) and plasminogen activator inhibitor (SERPINE1) in cultured bovine pulmonary artery endothelial cells. Dose-dependently inhibits collagen-induced platelet aggregation. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Cellular Component |
| extracellular region |
| Molecular Function |
| toxin activity |
| serine-type endopeptidase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 28397.395 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P05620 |
| UniProtKB/Swiss-Prot Entry Name
| VSP1_PROFL |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Deshimaru M, Ogawa T, Nakashima K, Nobuhisa I, Chijiwa T, Shimohigashi Y, Fukumaki Y, Niwa M, Yamashina I, Hattori S, Ohno M: Accelerated evolution of crotalinae snake venom gland serine proteases. FEBS Lett. 1996 Nov 11;397(1):83-8. doi: 10.1016/s0014-5793(96)01144-1. [PubMed:8941719 ]
- Sunagawa M, Nakamura M, Kosugi T: Cloning of habutobin cDNA and antithrombotic activity of recombinant protein. Biochem Biophys Res Commun. 2007 Nov 3;362(4):899-904. doi: 10.1016/j.bbrc.2007.08.103. Epub 2007 Aug 27. [PubMed:17803962 ]
- Shieh TC, Kawabata S, Kihara H, Ohno M, Iwanaga S: Amino acid sequence of a coagulant enzyme, flavoxobin, from Trimeresurus flavoviridis venom. J Biochem. 1988 Apr;103(4):596-605. doi: 10.1093/oxfordjournals.jbchem.a122313. [PubMed:3170503 ]
- Yamamoto C, Tsuru D, Oda-Ueda N, Ohno M, Hattori S, Kim ST: Flavoxobin, a serine protease from Trimeresurus flavoviridis (habu snake) venom, independently cleaves Arg726-Ser727 of human C3 and acts as a novel, heterologous C3 convertase. Immunology. 2002 Sep;107(1):111-7. doi: 10.1046/j.1365-2567.2002.01490.x. [PubMed:12225369 ]
- Shieh TC, Tanaka S, Kihara H, Ohno M, Makisumi S: Purification and characterization of a coagulant enzyme from Trimeresurus flavoviridis venom. J Biochem. 1985 Sep;98(3):713-21. doi: 10.1093/oxfordjournals.jbchem.a135329. [PubMed:3910643 ]
- Nakamura M, Kinjoh K, Miyagi C, Oka U, Sunagawa M, Yamashita S, Kosugi T: Pharmacokinetics of habutobin in rabbits. Toxicon. 1995 Sep;33(9):1201-6. doi: 10.1016/0041-0101(95)00050-v. [PubMed:8585090 ]
- Oka U, Nakamura M, Kinjoh K, Kosugi T: Alpha 2-macroglobulin of rabbits inhibits the habutobin activity. Toxicon. 1996 Aug;34(8):903-12. doi: 10.1016/0041-0101(96)00032-3. [PubMed:8875777 ]
- Kinjoh K, Kosugi T, Nakamura M, Hanashiro K, Sunagawa M, Tokeshi Y, Eguchi Y: Habutobin splits the Arg16-Gly17 bond in the A alpha chain of rabbit fibrinogen. Thromb Haemost. 1997 Jun;77(6):1127-8. [PubMed:9241744 ]
- Nejime T, Kinjoh K, Nakamura M, Hanashiro K, Sunagawa M, Eguchi Y, Kosugi T: Habutobin recognizes Thr(7) in the sequence of fibrinopeptide A of rabbit fibrinogen. Toxicon. 2000 Aug;38(8):1029-41. doi: 10.1016/s0041-0101(99)00215-9. [PubMed:10708795 ]
|
