| Identification |
|---|
| HMDB Protein ID
| HMDBP13402 |
| Secondary Accession Numbers
| None |
| Name
| Fibronectin-binding protein A |
| Synonyms
|
- FnbpA
|
| Gene Name
| FNBA |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Possesses multiple, substituting fibronectin (Fn) binding regions, each capable of conferring adherence to both soluble and immobilized forms of Fn. This confers to S.aureus the ability to invade endothelial cells both in vivo and in vitro, without requiring additional factors, although in a slow and inefficient way through actin rearrangements in host cells. This invasion process is mediated by integrin alpha-5/beta-1. Promotes bacterial attachment to both soluble and immobilized forms of fibrinogen (Fg) by means of a unique binding site localized within the 17 C-terminal residues of the gamma-chain of human Fg. Both plasma proteins (Fn and Fg) function as a bridge between bacterium and host cell. Promotes attachment to immobilized elastin peptides in a dose-dependent and saturable manner. Promotes attachment to both full-length and segments of immobilized human tropoelastin at multiple sites in a dose and pH-dependent manner. Promotes adherence to and aggregation of activated platelets independently of other S.aureus surface molecules. Is a critical mediator implicated in the induction of experimental endocarditis in rats with catheter-induced aortic vegetations, promoting both colonization and persistence of the bacterium into the host. |
| Pathways
|
- Bacterial invasion of epithelial cells
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| cell adhesion |
| pathogenesis |
| aggregation of unicellular organisms |
| Cellular Component |
| extracellular region |
| cell wall |
| Molecular Function |
| fibrinogen binding |
| fibronectin binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 1018 |
| Molecular Weight
| 111780.135 |
| Theoretical pI
| 4.711 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P14738 |
| UniProtKB/Swiss-Prot Entry Name
| FNBA_STAA8 |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Roche FM, Downer R, Keane F, Speziale P, Park PW, Foster TJ: The N-terminal A domain of fibronectin-binding proteins A and B promotes adhesion of Staphylococcus aureus to elastin. J Biol Chem. 2004 Sep 10;279(37):38433-40. doi: 10.1074/jbc.M402122200. Epub 2004 Jul 2. [PubMed:15234962 ]
- Signas C, Raucci G, Jonsson K, Lindgren PE, Anantharamaiah GM, Hook M, Lindberg M: Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptides. Proc Natl Acad Sci U S A. 1989 Jan;86(2):699-703. doi: 10.1073/pnas.86.2.699. [PubMed:2521391 ]
- Saravia-Otten P, Muller HP, Arvidson S: Transcription of Staphylococcus aureus fibronectin binding protein genes is negatively regulated by agr and an agr-independent mechanism. J Bacteriol. 1997 Sep;179(17):5259-63. doi: 10.1128/jb.179.17.5259-5263.1997. [PubMed:9286974 ]
- Wann ER, Gurusiddappa S, Hook M: The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus is a bifunctional protein that also binds to fibrinogen. J Biol Chem. 2000 May 5;275(18):13863-71. doi: 10.1074/jbc.275.18.13863. [PubMed:10788510 ]
- Massey RC, Kantzanou MN, Fowler T, Day NP, Schofield K, Wann ER, Berendt AR, Hook M, Peacock SJ: Fibronectin-binding protein A of Staphylococcus aureus has multiple, substituting, binding regions that mediate adherence to fibronectin and invasion of endothelial cells. Cell Microbiol. 2001 Dec;3(12):839-51. doi: 10.1046/j.1462-5822.2001.00157.x. [PubMed:11736995 ]
- Dunman PM, Murphy E, Haney S, Palacios D, Tucker-Kellogg G, Wu S, Brown EL, Zagursky RJ, Shlaes D, Projan SJ: Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci. J Bacteriol. 2001 Dec;183(24):7341-53. doi: 10.1128/JB.183.24.7341-7353.2001. [PubMed:11717293 ]
- Mazmanian SK, Ton-That H, Su K, Schneewind O: An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2293-8. doi: 10.1073/pnas.032523999. Epub 2002 Feb 5. [PubMed:11830639 ]
- Kruger RG, Otvos B, Frankel BA, Bentley M, Dostal P, McCafferty DG: Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA. Biochemistry. 2004 Feb 17;43(6):1541-51. doi: 10.1021/bi035920j. [PubMed:14769030 ]
- Heilmann C, Niemann S, Sinha B, Herrmann M, Kehrel BE, Peters G: Staphylococcus aureus fibronectin-binding protein (FnBP)-mediated adherence to platelets, and aggregation of platelets induced by FnBPA but not by FnBPB. J Infect Dis. 2004 Jul 15;190(2):321-9. doi: 10.1086/421914. Epub 2004 Jun 21. [PubMed:15216468 ]
- Entenza JM, Moreillon P, Senn MM, Kormanec J, Dunman PM, Berger-Bachi B, Projan S, Bischoff M: Role of sigmaB in the expression of Staphylococcus aureus cell wall adhesins ClfA and FnbA and contribution to infectivity in a rat model of experimental endocarditis. Infect Immun. 2005 Feb;73(2):990-8. doi: 10.1128/IAI.73.2.990-998.2005. [PubMed:15664942 ]
- Schroder A, Schroder B, Roppenser B, Linder S, Sinha B, Fassler R, Aepfelbacher M: Staphylococcus aureus fibronectin binding protein-A induces motile attachment sites and complex actin remodeling in living endothelial cells. Mol Biol Cell. 2006 Dec;17(12):5198-210. doi: 10.1091/mbc.e06-05-0463. Epub 2006 Oct 4. [PubMed:17021255 ]
- Keane FM, Clarke AW, Foster TJ, Weiss AS: The N-terminal A domain of Staphylococcus aureus fibronectin-binding protein A binds to tropoelastin. Biochemistry. 2007 Jun 19;46(24):7226-32. doi: 10.1021/bi700454x. Epub 2007 May 22. [PubMed:17516661 ]
- Keane FM, Loughman A, Valtulina V, Brennan M, Speziale P, Foster TJ: Fibrinogen and elastin bind to the same region within the A domain of fibronectin binding protein A, an MSCRAMM of Staphylococcus aureus. Mol Microbiol. 2007 Feb;63(3):711-23. doi: 10.1111/j.1365-2958.2006.05552.x. [PubMed:17302800 ]
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