| Identification |
|---|
| HMDB Protein ID
| HMDBP13377 |
| Secondary Accession Numbers
| None |
| Name
| Hydroperoxide isomerase ALOXE3 |
| Synonyms
|
- Epidermis-type lipoxygenase 3
- Hydroperoxy dehydratase ALOXE3
- Hydroperoxy icosatetraenoate dehydratase
- Hydroperoxy icosatetraenoate isomerase
- Epidermal LOX-3
- e-LOX-3
- eLOX-3
|
| Gene Name
| ALOXE3 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced lipoxygenases activity (PubMed:17045234). The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols and ketones (PubMed:17045234). In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (By similarity). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:22832496). In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites (By similarity). Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG (PubMed:20530198). Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia (By similarity). |
| Pathways
|
- hydroperoxy eicosatetraenoic acid biosynthesis
- sphingolipid metabolism
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| lipoxygenase pathway |
| peroxisome proliferator activated receptor signaling pathway |
| establishment of skin barrier |
| hepoxilin biosynthetic process |
| sensory perception of pain |
| fat cell differentiation |
| arachidonic acid metabolic process |
| ceramide biosynthetic process |
| sphingolipid metabolic process |
| linoleic acid metabolic process |
| Cellular Component |
| cytoplasm |
| Molecular Function |
| oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen |
| catalytic activity |
| hepoxilin A3 synthase activity |
| hydroperoxy icosatetraenoate dehydratase activity |
| hydroperoxy icosatetraenoate isomerase activity |
| intramolecular transferase activity, transferring hydroxy groups |
| iron ion binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 711 |
| Molecular Weight
| 80471.67 |
| Theoretical pI
| 6.8 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| Q9WV07 |
| UniProtKB/Swiss-Prot Entry Name
| LOXE3_MOUSE |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Church DM, Goodstadt L, Hillier LW, Zody MC, Goldstein S, She X, Bult CJ, Agarwala R, Cherry JL, DiCuccio M, Hlavina W, Kapustin Y, Meric P, Maglott D, Birtle Z, Marques AC, Graves T, Zhou S, Teague B, Potamousis K, Churas C, Place M, Herschleb J, Runnheim R, Forrest D, Amos-Landgraf J, Schwartz DC, Cheng Z, Lindblad-Toh K, Eichler EE, Ponting CP: Lineage-specific biology revealed by a finished genome assembly of the mouse. PLoS Biol. 2009 May 5;7(5):e1000112. doi: 10.1371/journal.pbio.1000112. Epub 2009 May 26. [PubMed:19468303 ]
- Kinzig A, Heidt M, Furstenberger G, Marks F, Krieg P: cDNA cloning, genomic structure, and chromosomal localization of a novel murine epidermis-type lipoxygenase. Genomics. 1999 Jun 1;58(2):158-64. doi: 10.1006/geno.1999.5816. [PubMed:10366447 ]
- Yu Z, Schneider C, Boeglin WE, Brash AR: Human and mouse eLOX3 have distinct substrate specificities: implications for their linkage with lipoxygenases in skin. Arch Biochem Biophys. 2006 Nov 15;455(2):188-96. doi: 10.1016/j.abb.2006.09.002. Epub 2006 Sep 25. [PubMed:17045234 ]
- Hallenborg P, Jorgensen C, Petersen RK, Feddersen S, Araujo P, Markt P, Langer T, Furstenberger G, Krieg P, Koppen A, Kalkhoven E, Madsen L, Kristiansen K: Epidermis-type lipoxygenase 3 regulates adipocyte differentiation and peroxisome proliferator-activated receptor gamma activity. Mol Cell Biol. 2010 Aug;30(16):4077-91. doi: 10.1128/MCB.01806-08. Epub 2010 Jun 7. [PubMed:20530198 ]
- Krieg P, Rosenberger S, de Juanes S, Latzko S, Hou J, Dick A, Kloz U, van der Hoeven F, Hausser I, Esposito I, Rauh M, Schneider H: Aloxe3 knockout mice reveal a function of epidermal lipoxygenase-3 as hepoxilin synthase and its pivotal role in barrier formation. J Invest Dermatol. 2013 Jan;133(1):172-80. doi: 10.1038/jid.2012.250. Epub 2012 Jul 26. [PubMed:22832496 ]
|
