| Identification |
|---|
| HMDB Protein ID
| HMDBP13376 |
| Secondary Accession Numbers
| None |
| Name
| Arachidonate 12-lipoxygenase, 12R-type |
| Synonyms
|
- 12R-LOX
- 12R-lipoxygenase
- Epidermis-type lipoxygenase 12
- Epidermis-type lipoxygenase 2
- e-LOX 2
|
| Gene Name
| ALOX12B |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:16129665). Does not convert arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE (PubMed:10100631, PubMed:11256953). In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:17403930, PubMed:17429434, PubMed:21558561). May also play a role in the regulation of the expression of airway mucins (By similarity). |
| Pathways
|
- Arachidonic acid metabolism
- hydroperoxy eicosatetraenoic acid biosynthesis
- Serotonergic synapse
- sphingolipid metabolism
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| protein lipidation |
| positive regulation of mucus secretion |
| linoleic acid metabolic process |
| positive regulation of gene expression |
| establishment of skin barrier |
| positive regulation of MAPK cascade |
| sphingolipid metabolic process |
| ceramide biosynthetic process |
| arachidonic acid metabolic process |
| hepoxilin biosynthetic process |
| lipoxygenase pathway |
| lipid oxidation |
| Cellular Component |
| perinuclear region of cytoplasm |
| cytosol |
| Molecular Function |
| linoleate 9S-lipoxygenase activity |
| arachidonate 12-lipoxygenase activity |
| oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen |
| arachidonate 8(R)-lipoxygenase activity |
| catalytic activity |
| iron ion binding |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 701 |
| Molecular Weight
| 80576.82 |
| Theoretical pI
| 6.709 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| O70582 |
| UniProtKB/Swiss-Prot Entry Name
| LX12B_MOUSE |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Sun D, McDonnell M, Chen XS, Lakkis MM, Li H, Isaacs SN, Elsea SH, Patel PI, Funk CD: Human 12(R)-lipoxygenase and the mouse ortholog. Molecular cloning, expression, and gene chromosomal assignment. J Biol Chem. 1998 Dec 11;273(50):33540-7. [PubMed:9837935 ]
- Krieg P, Kinzig A, Heidt M, Marks F, Furstenberger G: cDNA cloning of a 8-lipoxygenase and a novel epidermis-type lipoxygenase from phorbol ester-treated mouse skin. Biochim Biophys Acta. 1998 Mar 6;1391(1):7-12. doi: 10.1016/s0005-2760(97)00214-2. [PubMed:9518531 ]
- Krieg P, Siebert M, Kinzig A, Bettenhausen R, Marks F, Furstenberger G: Murine 12(R)-lipoxygenase: functional expression, genomic structure and chromosomal localization. FEBS Lett. 1999 Mar 5;446(1):142-8. doi: 10.1016/s0014-5793(99)00196-9. [PubMed:10100631 ]
- Siebert M, Krieg P, Lehmann WD, Marks F, Furstenberger G: Enzymic characterization of epidermis-derived 12-lipoxygenase isoenzymes. Biochem J. 2001 Apr 1;355(Pt 1):97-104. doi: 10.1042/0264-6021:3550097. [PubMed:11256953 ]
- Meruvu S, Walther M, Ivanov I, Hammarstrom S, Furstenberger G, Krieg P, Reddanna P, Kuhn H: Sequence determinants for the reaction specificity of murine (12R)-lipoxygenase: targeted substrate modification and site-directed mutagenesis. J Biol Chem. 2005 Nov 4;280(44):36633-41. doi: 10.1074/jbc.M508260200. Epub 2005 Aug 29. [PubMed:16129665 ]
- Epp N, Furstenberger G, Muller K, de Juanes S, Leitges M, Hausser I, Thieme F, Liebisch G, Schmitz G, Krieg P: 12R-lipoxygenase deficiency disrupts epidermal barrier function. J Cell Biol. 2007 Apr 9;177(1):173-82. doi: 10.1083/jcb.200612116. Epub 2007 Apr 2. [PubMed:17403930 ]
- Zheng Y, Yin H, Boeglin WE, Elias PM, Crumrine D, Beier DR, Brash AR: Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope. J Biol Chem. 2011 Jul 8;286(27):24046-56. doi: 10.1074/jbc.M111.251496. Epub 2011 May 10. [PubMed:21558561 ]
|
