| Identification |
|---|
| HMDB Protein ID
| HMDBP12683 |
| Secondary Accession Numbers
| None |
| Name
| 7beta-hydroxysteroid dehydrogenase |
| Synonyms
|
- 7beta-HSDH
- NADP-dependent 7beta-hydroxysteroid dehydrogenase
|
| Gene Name
| (KEGG) 7BETA-HYDROXYSTEROID DEHYDROGENASE (NADP+) (EC:1.1.1.201) |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| 7beta-hydroxysteroid dehydrogenase that catalyzes the reduction of the 7-oxo group of 7-oxo-lithocholate (7-oxo-LCA), to yield ursodeoxycholate (UDCA). As R.gnavus is a common core bacterium of the human gut microbiota, this enzyme contributes to the formation of UDCA in the human colon. UDCA is regarded as a chemopreventive beneficial secondary bile acid due to its low hydrophobicity; it protects hepatocytes and bile duct epithelial cells against necrosis and apoptosis induced by more hydrophobic secondary bile acids like deoxycholate (DCA). This enzyme is also able to catalyze the reverse reaction in vitro, i.e. the oxidation of the 7beta-hydroxy group of UDCA to 7-oxo-LCA, but much less efficiently than the reduction reaction, and this oxidation reaction is not observed in vivo. It is specific for NADPH/NADP(+) as the electron acceptor/donor since the activity observed with NADH/NAD(+) is less than 0.5% of that assayed using NADPH/NADP(+) in both directions. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| bile acid catabolic process |
| lipid catabolic process |
| Molecular Function |
| nucleotide binding |
| 7-beta-hydroxysteroid dehydrogenase (NADP+) activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 263 |
| Molecular Weight
| 29289.23 |
| Theoretical pI
| 5.124 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| R9UAM1 |
| UniProtKB/Swiss-Prot Entry Name
| HSDHB_RUMGN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Lee JY, Arai H, Nakamura Y, Fukiya S, Wada M, Yokota A: Contribution of the 7beta-hydroxysteroid dehydrogenase from Ruminococcus gnavus N53 to ursodeoxycholic acid formation in the human colon. J Lipid Res. 2013 Nov;54(11):3062-9. doi: 10.1194/jlr.M039834. Epub 2013 Jun 1. [PubMed:23729502 ]
|
