| Identification |
|---|
| HMDB Protein ID
| HMDBP12674 |
| Secondary Accession Numbers
| None |
| Name
| 7beta-hydroxysteroid dehydrogenase |
| Synonyms
|
- 7beta-HSDH
- NADP-dependent 7beta-hydroxysteroid dehydrogenase
|
| Gene Name
| Not Available |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| 7beta-hydroxysteroid dehydrogenase that catalyzes the reduction of the 7-oxo group of 7-oxo-lithocholate (7-oxo-LCA), to yield ursodeoxycholate (UDCA) (PubMed:21181147, PubMed:6954878, PubMed:27006087). As C.aerofaciens is an intestinal bacterium, this enzyme probably contributes to the formation of UDCA in the human colon. UDCA is regarded as a chemopreventive beneficial secondary bile acid due to its low hydrophobicity; it protects hepatocytes and bile duct epithelial cells against necrosis and apoptosis induced by more hydrophobic secondary bile acids like deoxycholate (DCA) (Probable). This enzyme is also able to catalyze the reverse reaction, i.e. the oxidation of the 7beta-hydroxy group of UDCA to 7-oxo-LCA (PubMed:21181147, PubMed:6954878). To a lesser extent, is also active on the taurine- and glycine-conjugates of ursodeoxycholate. It is specific for NADPH/NADP(+) as the electron acceptor/donor since it is not active with NADH/NAD(+) (PubMed:6954878). In the presence of NADPH, 7beta-HSDH can also reduce dehydrocholate (PubMed:21181147). And is also able to oxidize ursocholate (PubMed:27006087). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| bile acid catabolic process |
| lipid catabolic process |
| Molecular Function |
| nucleotide binding |
| 7-beta-hydroxysteroid dehydrogenase (NADP+) activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 28752.64 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| A4ECA9 |
| UniProtKB/Swiss-Prot Entry Name
| HSDHB_COLAA |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Hirano S, Masuda N: Characterization of NADP-dependent 7 beta-hydroxysteroid dehydrogenases from Peptostreptococcus productus and Eubacterium aerofaciens. Appl Environ Microbiol. 1982 May;43(5):1057-63. doi: 10.1128/aem.43.5.1057-1063.1982. [PubMed:6954878 ]
- Liu L, Aigner A, Schmid RD: Identification, cloning, heterologous expression, and characterization of a NADPH-dependent 7beta-hydroxysteroid dehydrogenase from Collinsella aerofaciens. Appl Microbiol Biotechnol. 2011 Apr;90(1):127-35. doi: 10.1007/s00253-010-3052-y. Epub 2010 Dec 23. [PubMed:21181147 ]
- Savino S, Ferrandi EE, Forneris F, Rovida S, Riva S, Monti D, Mattevi A: Structural and biochemical insights into 7beta-hydroxysteroid dehydrogenase stereoselectivity. Proteins. 2016 Jun;84(6):859-65. doi: 10.1002/prot.25036. Epub 2016 Apr 6. [PubMed:27006087 ]
- Wang R, Wu J, Jin DK, Chen Y, Lv Z, Chen Q, Miao Q, Huo X, Wang F: Structure of NADP(+)-bound 7beta-hydroxysteroid dehydrogenase reveals two cofactor-binding modes. Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):246-252. doi: 10.1107/S2053230X17004460. Epub 2017 Apr 26. [PubMed:28471355 ]
|
