| Identification |
|---|
| HMDB Protein ID
| HMDBP12667 |
| Secondary Accession Numbers
| None |
| Name
| 3alpha-hydroxy bile acid-CoA-ester 3-dehydrogenase 2 |
| Synonyms
|
- 3alpha-hydroxysteroid dehydrogenase 2
- Bile acid-inducible protein BaiA2
- 3alpha-HSDH 2
|
| Gene Name
| BAIA2 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Involved in the multi-step bile acid 7alpha-dehydroxylation pathway that transforms primary bile acids to secondary bile acids in the human gut (PubMed:23836456, PubMed:16299351). Catalyzes the oxidation of C3-hydroxyl group of CoA conjugated bile acids generating a C3-oxo bile acid intermediate. Can use choloyl-CoA, chenodeoxycholoyl-CoA, deoxycholoyl-CoA, and lithocholoyl-CoA as substrates with similar efficiency. Highly prefers NAD over NADP as cosubstrate. Also catalyzes the reverse reactions; in vitro, the preferred direction of reaction depends on the pH. Has very little activity with unconjugated (non-CoA) bile acid substrates (PubMed:23836456). |
| Pathways
|
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| bile acid biosynthetic process |
| protein homotetramerization |
| bile acid metabolic process |
| response to bile acid |
| Molecular Function |
| bile acid binding |
| NAD+ binding |
| steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 26538.29 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| P19337 |
| UniProtKB/Swiss-Prot Entry Name
| BAIA2_CLOSV |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Mallonee DH, White WB, Hylemon PB: Cloning and sequencing of a bile acid-inducible operon from Eubacterium sp. strain VPI 12708. J Bacteriol. 1990 Dec;172(12):7011-9. doi: 10.1128/jb.172.12.7011-7019.1990. [PubMed:2254270 ]
- Gopal-Srivastava R, Mallonee DH, White WB, Hylemon PB: Multiple copies of a bile acid-inducible gene in Eubacterium sp. strain VPI 12708. J Bacteriol. 1990 Aug;172(8):4420-6. doi: 10.1128/jb.172.8.4420-4426.1990. [PubMed:2376563 ]
- White WB, Franklund CV, Coleman JP, Hylemon PB: Evidence for a multigene family involved in bile acid 7-dehydroxylation in Eubacterium sp. strain VPI 12708. J Bacteriol. 1988 Oct;170(10):4555-61. doi: 10.1128/jb.170.10.4555-4561.1988. [PubMed:3170477 ]
- Bhowmik S, Jones DH, Chiu HP, Park IH, Chiu HJ, Axelrod HL, Farr CL, Tien HJ, Agarwalla S, Lesley SA: Structural and functional characterization of BaiA, an enzyme involved in secondary bile acid synthesis in human gut microbe. Proteins. 2014 Feb;82(2):216-29. doi: 10.1002/prot.24353. Epub 2013 Oct 17. [PubMed:23836456 ]
|
