| Identification |
|---|
| HMDB Protein ID
| HMDBP12623 |
| Secondary Accession Numbers
| None |
| Name
| 16S rRNA (adenine(1408)-N(1))-methyltransferase |
| Synonyms
|
- 16S rRNA m1A1408 methyltransferase
|
| Gene Name
| NPMA |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Specifically methylates the N(1) position of adenine 1408 in 16S rRNA. Confers resistance to various aminoglycosides, including kanamycin, neomycin, apramycin, ribostamycin and gentamicin. Methylates only fully assembled 30S subunits. |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| response to antibiotic |
| Molecular Function |
| tRNA (guanine-N7-)-methyltransferase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 219 |
| Molecular Weight
| 24886.29 |
| Theoretical pI
| 8.614 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| A8C927 |
| UniProtKB/Swiss-Prot Entry Name
| NPMA_ECOLX |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Zelinskaya N, Rankin CR, Macmaster R, Savic M, Conn GL: Expression, purification and crystallization of adenosine 1408 aminoglycoside-resistance rRNA methyltransferases for structural studies. Protein Expr Purif. 2011 Jan;75(1):89-94. doi: 10.1016/j.pep.2010.07.005. Epub 2010 Jul 25. [PubMed:20667473 ]
- Macmaster R, Zelinskaya N, Savic M, Rankin CR, Conn GL: Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria. Nucleic Acids Res. 2010 Nov;38(21):7791-9. doi: 10.1093/nar/gkq627. Epub 2010 Jul 17. [PubMed:20639535 ]
- Wachino J, Shibayama K, Kurokawa H, Kimura K, Yamane K, Suzuki S, Shibata N, Ike Y, Arakawa Y: Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides. Antimicrob Agents Chemother. 2007 Dec;51(12):4401-9. doi: 10.1128/AAC.00926-07. Epub 2007 Sep 17. [PubMed:17875999 ]
- Husain N, Obranic S, Koscinski L, Seetharaman J, Babic F, Bujnicki JM, Maravic-Vlahovicek G, Sivaraman J: Structural basis for the methylation of A1408 in 16S rRNA by a panaminoglycoside resistance methyltransferase NpmA from a clinical isolate and analysis of the NpmA interactions with the 30S ribosomal subunit. Nucleic Acids Res. 2011 Mar;39(5):1903-18. doi: 10.1093/nar/gkq1033. Epub 2010 Nov 9. [PubMed:21062819 ]
|
