| Identification |
|---|
| HMDB Protein ID
| HMDBP12358 |
| Secondary Accession Numbers
| None |
| Name
| Pullulanase A |
| Synonyms
|
- Alpha-dextrin endo-1,6-alpha-glucosidase
- Pullulan 6-glucanohydrolase
|
| Gene Name
| SPUA |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Virulence factor (By similarity). Involved in the degradation of glycogen of the mammalian host cells (PubMed:21565699). Hydrolyzes the alpha-1,6-branchpoints of glycogen (PubMed:20497336, PubMed:21565699). Hydrolyzes pullulan. Does not hydrolyze dextran (PubMed:20497336). Binds to mouse lung alveolar type II cells that are rich in glycogen stores. Is an alpha-glucan-specific carbohydrate-binding protein, which binds to amylose (pure alpha-(1,4)-linked glucose), amylopectin (alpha-(1,4)-linked glucose with alpha-(1,6) branch points), pullulan (linear polymer of mixed alpha-(1,4)- and alpha-(1,6)-linked glucose) and glycogen (similar to amylopectin with more frequent alpha-(1,6) branch points) in vitro. Does not bind to dextran (a linear polymer of alpha-(1,6)-linked glucose) (PubMed:17187076). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| pathogenesis |
| alpha-glucan biosynthetic process |
| Cellular Component |
| cell surface |
| extracellular region |
| cell wall |
| Molecular Function |
| calcium ion binding |
| polysaccharide binding |
| amylopectin binding |
| glycogen binding |
| limit dextrinase activity |
| pullulan binding |
| pullulanase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| 1280 |
| Molecular Weight
| 142618.275 |
| Theoretical pI
| 5.694 |
| Pfam Domain Function
|
|
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| A0A0H2UNG0 |
| UniProtKB/Swiss-Prot Entry Name
| PULA_STRPN |
| PDB IDs
|
|
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Tettelin H, Nelson KE, Paulsen IT, Eisen JA, Read TD, Peterson S, Heidelberg J, DeBoy RT, Haft DH, Dodson RJ, Durkin AS, Gwinn M, Kolonay JF, Nelson WC, Peterson JD, Umayam LA, White O, Salzberg SL, Lewis MR, Radune D, Holtzapple E, Khouri H, Wolf AM, Utterback TR, Hansen CL, McDonald LA, Feldblyum TV, Angiuoli S, Dickinson T, Hickey EK, Holt IE, Loftus BJ, Yang F, Smith HO, Venter JC, Dougherty BA, Morrison DA, Hollingshead SK, Fraser CM: Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science. 2001 Jul 20;293(5529):498-506. doi: 10.1126/science.1061217. [PubMed:11463916 ]
- Abbott DW, Higgins MA, Hyrnuik S, Pluvinage B, Lammerts van Bueren A, Boraston AB: The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniae. Mol Microbiol. 2010 Jul 1;77(1):183-99. doi: 10.1111/j.1365-2958.2010.07199.x. Epub 2010 May 19. [PubMed:20497336 ]
- van Bueren AL, Higgins M, Wang D, Burke RD, Boraston AB: Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors. Nat Struct Mol Biol. 2007 Jan;14(1):76-84. doi: 10.1038/nsmb1187. Epub 2006 Dec 24. [PubMed:17187076 ]
- Lammerts van Bueren A, Ficko-Blean E, Pluvinage B, Hehemann JH, Higgins MA, Deng L, Ogunniyi AD, Stroeher UH, El Warry N, Burke RD, Czjzek M, Paton JC, Vocadlo DJ, Boraston AB: The conformation and function of a multimodular glycogen-degrading pneumococcal virulence factor. Structure. 2011 May 11;19(5):640-51. doi: 10.1016/j.str.2011.03.001. [PubMed:21565699 ]
|
