| Identification |
|---|
| HMDB Protein ID
| HMDBP12313 |
| Secondary Accession Numbers
| None |
| Name
| Isopropyl malate synthase htyA |
| Synonyms
|
- L-homotyrosine biosynthetic cluster protein A
|
| Gene Name
| HTYA |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Not Available |
| Specific Function
| Isopropyl malate synthase; part of the gene cluster that mediates the de novo generation of L-homotyrosine from acetyl-CoA and 4-hydroxyphenyl-pyruvate (PubMed:22998630). L-homotyrosine is a building block of echinocandin B, a fungal lipidated cyclic hexapeptide that acts as an antifungal agent (PubMed:22998630). L-homotyrosine 4-hydroxyphenyl-pyruvate first undergoes an aldol-type condensation by htyA with the C-2 of acetyl-CoA followed by the release of CoA to form 2-(4-hydroxybenzyl)-malate (PubMed:22998630). This is followed by isomerization of 2-(4-hydroxy-benzyl)-malate to 3-(4-hydroxybenzyl)-malate by htyD (PubMed:22998630). Thereafter, 3-(4-hydroxybenzyl)-malate undergoes decarboxylation and oxidation to form 2-oxo-4-(4-hydroxybenzyl)butanoic acid, coupled to reduction of NAD(+) to NADH by htyC (PubMed:22998630). The product then undergoes transamination catalyzed by htyB to form L-homotyrosine (PubMed:22998630). |
| Pathways
|
Not Available
|
| Reactions
| Not Available |
| GO Classification
|
| Biological Process |
| carboxylic acid metabolic process |
| Molecular Function |
| 2-isopropylmalate synthase activity |
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| Not Available |
| Locus
| Not Available |
| SNPs
| Not Available |
| Gene Sequence
|
Not Available
|
| Protein Properties |
|---|
| Number of Residues
| Not Available |
| Molecular Weight
| 63713.165 |
| Theoretical pI
| Not Available |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
Not Available
|
| External Links |
|---|
| GenBank ID Protein
| Not Available |
| UniProtKB/Swiss-Prot ID
| K0E4E5 |
| UniProtKB/Swiss-Prot Entry Name
| HTYA_ASPRU |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| Not Available |
| GeneCard ID
| Not Available |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| - Cacho RA, Jiang W, Chooi YH, Walsh CT, Tang Y: Identification and characterization of the echinocandin B biosynthetic gene cluster from Emericella rugulosa NRRL 11440. J Am Chem Soc. 2012 Oct 10;134(40):16781-90. doi: 10.1021/ja307220z. Epub 2012 Oct 1. [PubMed:22998630 ]
- Mattay J, Houwaart S, Huttel W: Cryptic Production of trans-3-Hydroxyproline in Echinocandin B Biosynthesis. Appl Environ Microbiol. 2018 Mar 19;84(7). pii: AEM.02370-17. doi: 10.1128/AEM.02370-17. Print 2018 Apr 1. [PubMed:29352089 ]
|
