Human Metabolome Database: Showing Protein Protein-methionine sulfoxide oxidase MICAL2 (HMDBP08275)

Identification Biological properties Gene properties Protein properties External links References XML Show 5 metabolites

Identification

HMDB Protein ID

HMDBP08275

Secondary Accession Numbers

13987

Name

Protein-methionine sulfoxide oxidase MICAL2

Synonyms

Molecule interacting with CasL protein 2
MICAL-2

Gene Name

MICAL2

Protein Type

Unknown

Biological Properties

General Function

Involved in monooxygenase activity

Specific Function

Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (By similarity).

Pathways

Not Available

Reactions

[protein]-methionine + NADPH + Oxygen → [protein]-methionine-sulfoxide + NADP + Water

details

GO Classification

Biological Process
actin filament depolymerization
Cellular Component
cytoskeleton
cytoplasm
Function
cation binding
monooxygenase activity
oxidoreductase activity
zinc ion binding
transition metal ion binding
catalytic activity
binding
metal ion binding
ion binding
Molecular Function
metal ion binding
zinc ion binding
FAD binding
actin binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen
Process
metabolic process
oxidation reduction

Cellular Location

Cytoplasm
Cytoplasm
cytoskeleton

Gene Properties

Chromosome Location

Locus

11p15.3

SNPs

MICAL2

Gene Sequence

>3375 bp
ATGGGGGAAAACGAGGATGAGAAGCAGGCCCAGGCGGGGCAGGTTTTTGAGAACTTTGTC
CAGGCATCCACGTGCAAAGGTACCCTCCAGGCCTTCAACATTCTCACACGACACCTGGAC
CTAGACCCTCTGGACCACAGAAACTTTTATTCCAAGCTCAAGTCCAAGGTGACCACCTGG
AAAGCCAAAGCCCTGTGGTACAAATTGGATAAGCGTGGTTCCCACAAAGAGTATAAGCGA
GGGAAGTCGTGCACGAACACCAAGTGTCTCATAGTTGGGGGAGGACCCTGTGGCTTGCGC
ACTGCCATTGAACTTGCCTACCTGGGAGCCAAAGTGGTCGTGGTGGAGAAGAGGGACTCC
TTCTCCCGGAACAACGTGCTACACCTCTGGCCTTTCACCATCCATGACCTTCGGGGCCTG
GGAGCCAAGAAGTTCTATGGGAAGTTCTGTGCTGGCTCCATCGACCATATCAGTATTCGC
CAACTACAGCTCATCCTATTCAAGGTGGCCCTGATGCTGGGAGTTGAAATCCATGTGAAT
GTGGAGTTCGTGAAGGTTCTAGAGCCTCCTGAAGATCAAGAAAATCAAAAAATTGGCTGG
CGGGCAGAATTTCTCCCTACAGACCATTCTCTGTCGGAGTTTGAGTTTGACGTCATCATT
GGTGCCGATGGCCGCAGGAACACCCTGGAAGGGTTCAGAAGAAAAGAATTCCGTGGGAAG
CTGGCGATTGCCATCACCGCCAACTTCATAAACAGAAACAGCACAGCGGAAGCCAAGGTG
GAAGAGATTAGTGGTGTGGCTTTCATCTTCAATCAGAAATTTTTTCAGGACCTTAAAGAA
GAAACAGGCATAGATCTTGAGAACATTGTTTACTACAAGGACTGCACCCACTATTTTGTA
ATGACAGCCAAGAAGCAGAGCCTGCTCGACAAAGGTGTCATCATTAACGACTACATCGAC
ACAGAGATGCTGCTGTGTGCGGAGAACGTGAACCAAGACAACCTGCTATCCTATGCCCGG
GAAGCTGCAGACTTTGCCACCAACTACCAGCTGCCATCCTTAGACTTTGCCATGAACCAC
TATGGGCAGCCTGATGTGGCCATGTTTGACTTTACCTGCATGTATGCCTCAGAGAACGCG
GCCCTGGTGCGGGAGCGGCAGGCGCACCAGCTGCTCGTGGCCCTTGTGGGTGACAGCTTG
CTTGAGCCATTTTGGCCCATGGGTACAGGCTGTGCCCGTGGCTTCCTGGCAGCCTTTGAC
ACGGCATGGATGGTGAAGAGCTGGAACCAGGGCACCCCTCCCCTGGAGCTGCTGGCTGAA
AGGGAAAGTCTCTACCGGCTGTTACCTCAGACAACCCCGGAGAACATCAACAAGAACTTT
GAGCAGTACACGTTGGACCCAGGGACACGGTACCCAAACCTCAACTCACACTGTGTCAGG
CCCCATCAGGTGAAGCATTTGTATATCACTAAGGAGCTGGAGCACTACCCTCTCGAGAGA
CTGGGCTCGGTGAGGAGATCTGTCAACCTCTCCAGGAAGGAGTCAGATATCCGGCCCAGC
AAGCTCCTGACCTGGTGCCAGCAGCAGACAGAGGGCTACCAGCATGTCAACGTCACCGAC
CTGACCACATCCTGGCGCAGTGGGTTGGCCCTGTGTGCCATCATCCACCGCTTCCGGCCT
GAGCTCATCAACTTTGACTCTTTGAATGAAGATGATGCTGTGGAGAACAACCAGCTCGCA
TTTGATGTGGCCGAGCGAGAGTTTGGGATCCCTCCAGTGACCACGGGCAAAGAGATGGCA
TCTGCCCAGGAGCCTGACAAGCTCAGCATGGTCATGTACCTCTCCAAGTTCTACGAGCTC
TTCCGGGGCACCCCACTGAGGCCCGTGGATTCTTGGCGCAAAAACTATGGAGAAAATGCT
GACCTCAGCTTGGCCAAATCATCCATTTCTAATAACTATCTCAACCTCACATTTCCAAGG
AAGAGGACTCCACGGGTGGATGGTCAAACCGGAGAGAATGACATGAACAAACGGAGACGG
AAGGGCTTCACCAACCTGGACGAGCCTTCAAACTTTTCCAGCCGTAGCTTGGGCTCCAAT
CAAGAGTGTGGGAGCAGTAAGGAAGGTGGAAATCAGAACAAAGTCAAGTCCATGGCGAAT
CAGCTGCTGGCCAAGTTTGAGGAGAGCACTCGGAACCCCTCACTCATGAAGCAGGAACGC
CGTGTCTCAGGGATAGGTAAGCCGGTCCTGTGCTCTTCCTCCGGCCCTCCTGTTCACTCT
TGCTGCCCCAAGCCGGAGGAGGCCACACCCAGCCCATCACCTCCTCTGAAAAGGCAGTTC
CCCTCTGTGGTCGTGACGGGGCACGTGCTCAGAGAGCTCAAGCAAGTGTCTGCTGGCAGT
GAGTGCCTGAGCAGACCTTGGAGAGCCAGAGCCAAGTCTGACCTACAGCTGGGTGGGACA
GAAAATTTCGCTACCCTGCCTTCTACCCGCCCGAGGGCGCAGGCTCTTTCCGGGGTGCTG
TGGCGGCTGCAGCAAGTGGAGGAAAAGATTCTCCAGAAGAGGGCTCAGAACTTGGCCAAC
AGGGAATTTCACACAAAGAACATTAAGGAGAAGGCGGCTCACCTTGCCTCCATGTTTGGA
CACGGGGATTTCCCGCAGAATAAACTACTCTCTAAAGGCCTGTCTCATACTCATCCTCCA
TCTCCTCCCTCTCGCCTTCCGTCTCCTGATCCAGCTGCTTCTTCCTCTCCATCAACTGTT
GACTCTGCTTCTCCTGCCAGAAAGGAAAAGAAGTCACCTTCAGGGTTCCATTTTCATCCC
AGCCATTTGAGAACAGTGCATCCTCAGCTGACGGTAGGGAAAGTGTCCAGCGGAATAGGG
GCTGCAGCTGAAGTCCTGGTCAATCTGTACATGAATGATCACAGACCTAAGGCCCAGGCC
ACCTCTCCAGACCTGGAATCTATGCGAAAGTCATTTCCCCTTAACCTGGGAGGCAGCGAC
ACGTGTTACTTCTGTAAGAAACGTGTGTACGTGATGGAACGGCTGAGCGCCGAGGGCCAC
TTCTTCCACCGGGAGTGTTTCCGCTGCAGCATCTGTGCCACCACCTTGCGCCTGGCCGCC
TACACCTTTGACTGCGATGAAGGCAAATTTTACTGCAAGCCTCACTTCATTCACTGTAAA
ACCAATAGCAAACAACGGAAGAGACGGGCAGAGTTGAAGCAACAAAGAGAGGAGGAGGCA
ACATGGCAAGAGCAGGAAGCCCCTCGGAGAGACACTCCCACCGAAAGTTCTTGCGCAGTG
GCCGCCATTGGCACCCTGGAAGGCAGCCCCCCAGTTCATTTCAGCCTTCCAGTGCTACAC
CCACTTCTTGGCTGA

Protein Properties

Number of Residues

1124

Molecular Weight

126687.955

Theoretical pI

8.641

Pfam Domain Function

LIM (PF00412 )
FAD_binding_3 (PF01494 )
CH (PF00307 )

Signals

Not Available

Transmembrane Regions

Not Available

Protein Sequence

>Protein MICAL-2
MGENEDEKQAQAGQVFENFVQASTCKGTLQAFNILTRHLDLDPLDHRNFYSKLKSKVTTW
KAKALWYKLDKRGSHKEYKRGKSCTNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDS
FSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEIHVN
VEFVKVLEPPEDQENQKIGWRAEFLPTDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGK
LAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDCTHYFV
MTAKKQSLLDKGVIINDYIDTEMLLCAENVNQDNLLSYAREAADFATNYQLPSLDFAMNH
YGQPDVAMFDFTCMYASENAALVRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFD
TAWMVKSWNQGTPPLELLAERESLYRLLPQTTPENINKNFEQYTLDPGTRYPNLNSHCVR
PHQVKHLYITKELEHYPLERLGSVRRSVNLSRKESDIRPSKLLTWCQQQTEGYQHVNVTD
LTTSWRSGLALCAIIHRFRPELINFDSLNEDDAVENNQLAFDVAEREFGIPPVTTGKEMA
SAQEPDKLSMVMYLSKFYELFRGTPLRPVDSWRKNYGENADLSLAKSSISNNYLNLTFPR
KRTPRVDGQTGENDMNKRRRKGFTNLDEPSNFSSRSLGSNQECGSSKEGGNQNKVKSMAN
QLLAKFEESTRNPSLMKQERRVSGIGKPVLCSSSGPPVHSCCPKPEEATPSPSPPLKRQF
PSVVVTGHVLRELKQVSAGSECLSRPWRARAKSDLQLGGTENFATLPSTRPRAQALSGVL
WRLQQVEEKILQKRAQNLANREFHTKNIKEKAAHLASMFGHGDFPQNKLLSKGLSHTHPP
SPPSRLPSPDPAASSSPSTVDSASPARKEKKSPSGFHFHPSHLRTVHPQLTVGKVSSGIG
AAAEVLVNLYMNDHRPKAQATSPDLESMRKSFPLNLGGSDTCYFCKKRVYVMERLSAEGH
FFHRECFRCSICATTLRLAAYTFDCDEGKFYCKPHFIHCKTNSKQRKRRAELKQQREEEA
TWQEQEAPRRDTPTESSCAVAAIGTLEGSPPVHFSLPVLHPLLG

External Links

GenBank ID Protein

7662284

UniProtKB/Swiss-Prot ID

O94851

UniProtKB/Swiss-Prot Entry Name

MICA2_HUMAN

PDB IDs

2E9K

GenBank Gene ID

GeneCard ID

GenAtlas ID

HGNC ID

References

General References

Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007 Oct 31;8:399. [PubMed:17974005 ]
Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O: Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 1998 Oct 30;5(5):277-86. [PubMed:9872452 ]
Terman JR, Mao T, Pasterkamp RJ, Yu HH, Kolodkin AL: MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion. Cell. 2002 Jun 28;109(7):887-900. [PubMed:12110185 ]
Fischer J, Weide T, Barnekow A: The MICAL proteins and rab1: a possible link to the cytoskeleton? Biochem Biophys Res Commun. 2005 Mar 11;328(2):415-23. [PubMed:15694364 ]