| Identification |
|---|
| HMDB Protein ID
| HMDBP01705 |
| Secondary Accession Numbers
| |
| Name
| Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A |
| Synonyms
|
- Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
- GNT-V
- GlcNAc-T V
- Mannoside acetylglucosaminyltransferase 5
- N-acetylglucosaminyl-transferase V
|
| Gene Name
| MGAT5 |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in alpha-1,6-mannosyl-glycoprotein 6-beta-N-ac |
| Specific Function
| Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
|
| Pathways
|
- N-Glycan biosynthesis
- protein glycosylation
|
| Reactions
|
| Uridine diphosphate-N-acetylglucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R → Uridine 5'-diphosphate + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R |
details
|
| UDP-N-acetyl-D-glucosamine + → UDP + |
details
|
| UDP-N-acetyl-D-glucosamine + → UDP + |
details
|
|
| GO Classification
|
| Biological Process |
| post-translational protein modification |
| protein N-linked glycosylation via asparagine |
| Cellular Component |
| integral to membrane |
| Golgi membrane |
| Molecular Function |
| alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity |
|
| Cellular Location
|
- Golgi apparatus membrane
- Single-pass type II membrane protein
|
| Gene Properties |
|---|
| Chromosome Location
| 2 |
| Locus
| 2q21.3 |
| SNPs
| MGAT5 |
| Gene Sequence
|
>2226 bp
ATGGCTCTCTTCACTCCGTGGAAGTTGTCCTCTCAGAAGCTGGGCTTTTTCCTGGTGACT
TTTGGCTTCATTTGGGGTATGATGCTTCTGCACTTTACCATCCAGCAGCGAACTCAGCCT
GAAAGCAGCTCCATGCTGCGCGAGCAGATCCTGGACCTCAGCAAAAGGTACATCAAGGCA
CTGGCAGAAGAAAACAGGAATGTGGTGGATGGGCCATACGCTGGAGTCATGACAGCTTAT
GATCTGAAGAAAACCCTTGCTGTGTTATTAGATAACATTTTGCAGCGCATTGGCAAGTTG
GAGTCGAAGGTGGACAATCTTGTTGTCAATGGCACCGGAACAAACTCAACCAACTCCACT
ACAGCTGTTCCCAGCTTGGTTGCACTTGAGAAAATTAATGTGGCAGATATCATTAACGGA
GCTCAAGAAAAATGTGTATTGCCTCCTATGGACGGCTACCCTCACTGTGAGGGAAAGATC
AAGTGGATGAAAGACATGTGGCGTTCAGATCCCTGCTACGCAGACTATGGAGTGGATGGA
TCCACCTGCTCTTTTTTTATTTACCTCAGTGAGGTTGAAAATTGGTGTCCTCATTTACCT
TGGAGAGCAAAAAATCCCTACGAAGAAGCTGATCATAATTCATTGGCGGAAATTCGTACA
GATTTTAATATTCTCTACAGTATGATGAAAAAGCATGAAGAATTCCGGTGGATGAGACTA
CGGATCCGGCGAATGGCTGACGCATGGATCCAAGCAATCAAGTCCCTGGCAGAAAAGCAG
AACCTTGAAAAGAGAAAGCGGAAGAAAGTCCTCGTTCACCTGGGACTCCTGACCAAGGAA
TCTGGATTTAAGATTGCAGAGACAGCTTTCAGTGGTGGCCCTCTTGGTGAATTAGTTCAA
TGGAGTGATTTAATTACATCTCTGTACTTACTGGGCCATGACATTAGGATTTCAGCTTCA
CTGGCTGAGCTCAAGGAAATCATGAAGAAGGTTGTAGGAAACCGATCTGGCTGCCCAACT
GTAGGAGACAGAATTGTTGAGCTCATTTACATTGATATTGTAGGACTTGCTCAATTCAAG
AAAACTCTTGGACCATCCTGGGTTCATTACCAGTGCATGCTCCGAGTGCTGGATTCCTTT
GGAACAGAACCTGAGTTCAATCACGCAAATTATGCCCAATCGAAAGGCCACAAGACCCCT
TGGGGAAAATGGAATCTGAACCCTCAGCAGTTTTATACCATGTTCCCTCATACCCCAGAC
AACAGCTTTCTGGGGTTTGTGGTTGAGCAGCACCTGAACTCCAGTGATATCCACCACATT
AATGAAATCAAAAGGCAGAACCAGTCCCTTGTGTATGGCAAAGTGGATAGCTTCTGGAAG
AATAAGAAGATCTACTTGGACATTATTCACACATACATGGAAGTGCATGCAACTGTTTAT
GGCTCCAGCACAAAGAATATTCCCAGTTACGTGAAAAACCATGGTATCCTCAGTGGACGG
GACCTGCAGTTCCTTCTTCGAGAAACCAAGTTGTTTGTTGGACTTGGGTTCCCTTACGAG
GGCCCAGCTCCCCTGGAAGCTATCGCAAATGGATGTGCTTTTCTGAATCCCAAGTTCAAC
CCACCCAAAAGCAGCAAAAACACAGACTTCTTCATTGGCAAGCCAACACTGAGAGAGCTC
ACATCCCAGCATCCTTACGCTGAAGTTTTCATCGGGCGGCCACATGTGTGGACTGTTGAC
CTCAACAATCAGGAGGAAGTAGAGGATGCAGTGAAAGCCATCTTAAACCAGAAGATTGAG
CCATACATGCCATATGAATTTACGTGCGAGGGGATGCTACAGAGAATCAATGCTTTCATT
GAAAAACAGGACTTCTGCCATGGGCAAGTGATGTGGCCACCCCTCAGCGCCCTACAGGTC
AAGCTTGCTGAGCCCGGGCAGTCCTGCAAGCAGGTGTGCCAGGAGAGCCAGCTCATCTGC
GAGCCTTCTTTCTTCCAGCACCTCAACAAGGACAAGGACATGCTGAAGTACAAGGTGACC
TGCCAAAGCTCAGAGCTGGCCAAGGACATCCTGGTGCCCTCCTTTGACCCTAAGAATAAG
CACTGTGTGTTTCAAGGTGACCTCCTGCTCTTCAGCTGTGCAGGCGCCCACCCCAGGCAC
CAGAGGGTCTGCCCCTGCCGGGACTTCATCAAGGGCCAGGTGGCTCTCTGCAAAGACTGC
CTATAG
|
| Protein Properties |
|---|
| Number of Residues
| 741 |
| Molecular Weight
| 84542.02 |
| Theoretical pI
| 8.124 |
| Pfam Domain Function
|
Not Available |
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
MALFTPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSSMLREQILDLSKRYIKA
LAEENRNVVDGPYAGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVVNGTGTNSTNST
TAVPSLVALEKINVADIINGAQEKCVLPPMDGYPHCEGKIKWMKDMWRSDPCYADYGVDG
STCSFFIYLSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWMRL
RIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGELVQ
WSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQFK
KTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPD
NSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVY
GSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFN
PPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIE
PYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLIC
EPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRH
QRVCPCRDFIKGQVALCKDCL
|
| External Links |
|---|
| GenBank ID Protein
| 4545222 |
| UniProtKB/Swiss-Prot ID
| Q09328 |
| UniProtKB/Swiss-Prot Entry Name
| MGT5A_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| AF113921 |
| GeneCard ID
| MGAT5 |
| GenAtlas ID
| Not Available |
| HGNC ID
| HGNC:7049 |
| References |
|---|
| General References
| - Saito H, Nishikawa A, Gu J, Ihara Y, Soejima H, Wada Y, Sekiya C, Niikawa N, Taniguchi N: cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+. Biochem Biophys Res Commun. 1994 Jan 14;198(1):318-27. [PubMed:8292036 ]
- Park C, Jin UH, Lee YC, Cho TJ, Kim CH: Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B. Arch Biochem Biophys. 1999 Jul 15;367(2):281-8. [PubMed:10395745 ]
|
