| Identification |
|---|
| HMDB Protein ID
| HMDBP00867 |
| Secondary Accession Numbers
| |
| Name
| Alpha-mannosidase 2 |
| Synonyms
|
- AMan II
- Golgi alpha-mannosidase II
- Man II
- Mannosidase alpha class 2A member 1
- Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
|
| Gene Name
| MAN2A1 |
| Protein Type
| Unknown |
| Biological Properties |
|---|
| General Function
| Involved in catalytic activity |
| Specific Function
| Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
|
| Pathways
|
- N-Glycan biosynthesis
- protein glycosylation
|
| Reactions
|
|
| GO Classification
|
| Biological Process |
| vacuole organization |
| mannose metabolic process |
| N-glycan processing |
| respiratory gaseous exchange |
| mitochondrion organization |
| in utero embryonic development |
| protein N-linked glycosylation via asparagine |
| post-translational protein modification |
| lung alveolus development |
| liver development |
| Cellular Component |
| Golgi membrane |
| integral to membrane |
| Function |
| alpha-mannosidase activity |
| mannosidase activity |
| ion binding |
| hydrolase activity, hydrolyzing o-glycosyl compounds |
| zinc ion binding |
| cation binding |
| metal ion binding |
| binding |
| catalytic activity |
| hydrolase activity |
| transition metal ion binding |
| hydrolase activity, acting on glycosyl bonds |
| carbohydrate binding |
| Molecular Function |
| zinc ion binding |
| metal ion binding |
| carbohydrate binding |
| hydrolase activity, hydrolyzing N-glycosyl compounds |
| mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity |
| Process |
| carbohydrate metabolic process |
| primary metabolic process |
| small molecule metabolic process |
| metabolic process |
| alcohol metabolic process |
| monosaccharide metabolic process |
| hexose metabolic process |
| mannose metabolic process |
|
| Cellular Location
|
- Golgi apparatus membrane
- Single-pass type II membrane protein
|
| Gene Properties |
|---|
| Chromosome Location
| 5 |
| Locus
| 5q21-q22 |
| SNPs
| MAN2A1 |
| Gene Sequence
|
>3435 bp
ATGAAGTTAAGCCGCCAGTTCACCGTGTTCGGCAGTGCGATCTTCTGTGTGGTGATTTTC
TCGCTCTACCTGATGCTGGACCGGGGTCACTTAGACTACCCCAGGAACCCGCGCCGCGAG
GGCTCCTTCCCTCAGGGCCAGCTCTCAATGTTGCAAGAAAAAATAGACCATTTGGAGCGT
TTGCTAGCTGAGAATAATGAGATCATCTCAAATATTAGAGACTCAGTCATCAATTTGAGT
GAGTCTGTGGAGGATGGTCCGAAAAGTTCACAAAGCAATTTCAGCCAAGGTGCTGGCTCA
CATCTTCTGCCCTCACAATTATCCCTCTCAGTTGACACTGCAGACTGTCTGTTTGCTTCA
CAAAGTGGAAGTCACAATTCAGATGTGCAGATGTTGGATGTTTACAGTCTAATTTCTTTT
GACAATCCAGATGGTGGAGTTTGGAAGCAAGGATTTGACATTACTTATGAATCTAATGAA
TGGGACACTGAACCCCTTCAAGTCTTTGTGGTGCCTCATTCCCATAACGACCCAGGTTGG
TTGAAGACTTTCAATGACTACTTTAGAGACAAGACTCAGTATATTTTTAATAACATGGTC
CTAAAGCTGAAAGAAGACTCACGGAGGAAGTTTATTTGGTCTGAGATCTCTTACCTTTCA
AAGTGGTGGGATATTATAGATATTCAGAAGAAGGATGCTGTTAAAAGTTTAATAGAAAAT
GGTCAGCTTGAAATTGTGACAGGTGGCTGGGTTATGCCTGATGAAGCTACTCCACATTAT
TTTGCCTTAATTGATCAACTAATTGAAGGACATCAGTGGCTGGAAAATAATATAGGAGTG
AAACCTCGGTCCGGCTGGGCTATTGATCCCTTTGGACACTCACCAACAATGGCTTATCTT
CTAAACCGTGCTGGACTTTCTCACATGCTTATCCAGAGAGTTCATTATGCAGTTAAAAAA
CACTTTGCACTGCATAAAACATTGGAGTTTTTTTGGAGACAGAATTGGGATCTGGGATCT
GTCACAGATATTTTATGCCACATGATGCCCTTCTACAGCTATGACATCCCTCACACTTGT
GGACCTGATCCTAAAATATGCTGCCAGTTTGATTTTAAACGTCTTCCTGGAGGCAGATTT
GGTTGTCCCTGGGGAGTCCCCCCAGAAACAATACATCCTGGAAATGTCCAAAGCAGGGCT
CGGATGCTACTAGATCAGTACCGAAAGAAGTCAAAGCTTTTTCGTACCAAAGTTCTCCTG
GCTCCACTAGGAGATGATTTCCGCTACTGTGAATACACGGAATGGGATTTACAGTTTAAG
AATTATCAGCAGCTTTTTGATTATATGAATTCTCAGTCCAAGTTTAAAGTTAAGATACAG
TTTGGAACTTTATCAGATTTTTTTGATGCGCTGGATAAAGCAGATGAAACTCAGAGAGAC
AAGGGCCAATCGATGTTCCCTGTTTTAAGTGGAGATTTTTTCACTTATGCCGATCGAGAT
GATCATTACTGGAGTGGCTATTTTACATCCAGACCCTTTTACAAACGAATGGACAGAATC
ATGGAATCTCATTTAAGGGCTGCTGAAATTCTTTACTATTTCGCCCTGAGACAAGCTCAC
AAATACAAGATAAATAAATTTCTCTCATCATCACTTTACACGGCACTGACAGAAGCCAGA
AGGAATTTGGGACTGTTTCAACATCATGATGCTATCACAGGAACTGCAAAAGACTGGGTG
GTTGTGGATTATGGTACCAGACTTTTTCATTCGTTAATGGTTTTGGAGAAGATAATTGGA
AATTCTGCATTTCTTCTTATTTTGAAGGACAAACTCACATACGACTCTTACTCTCCTGAT
ACCTTCCTGGAGATGGATTTGAAACAAAAATCACAAGATTCTCTGCCACAAAAAAATATA
ATAAGGCTGAGTGCGGAGCCAAGGTACCTTGTGGTCTATAATCCTTTAGAACAAGACCGA
ATCTCGTTGGTCTCAGTCTATGTGAGTTCCCCGACAGTGCAAGTGTTCTCTGCTTCAGGA
AAACCTGTGGAAGTTCAAGTCAGCGCAGTTTGGGATACAGCAAATACTATTTCAGAAACA
GCCTATGAGATCTCTTTTCGAGCACATATACCGCCATTGGGACTGAAAGTGTATAAGATT
TTGGAATCAGCAAGTTCAAATTCACATTTAGCTGATTATGTCTTGTATAAGAATAAAGTA
GAAGATAGCGGAATTTTCACCATAAAGAATATGATAAATACTGAAGAAGGTATAACACTA
GAGAACTCCTTTGTTTTACTTCGGTTTGATCAAACTGGACTTATGAAGCAAATGATGACT
AAAGAAGATGGTAAACACCATGAAGTAAATGTGCAATTTTCATGGTATGGAACCACAATT
AAAAGAGACAAAAGTGGTGCCTACCTCTTCTTACCTGATGGTAATGCCAAGCCTTATGTT
TACACAACACCGCCCTTTGTCAGAGTGACACATGGAAGGATTTATTCGGAAGTGACTTGC
TTTTTTGACCATGTTACTCATAGAGTCCGACTATACCACATACAGGGAATAGAAGGACAG
TCTGTGGAAGTTTCCAATATTGTGGACATCCGAAAAGTATATAACCGTGAGATTGCAATG
AAAATTTCTTCTGATATAAAAAGCCAAAATAGATTTTATACTGACCTAAATGGGTACCAG
ATTCAACCTAGAATGACACTGAGCAAATTGCCTCTTCAAGCAAATGTCTATCCCATGACC
ACAATGGCCTATATCCAGGATGCCAAACATCGTTTGACACTGCTCTCTGCTCAGTCATTA
GGGGTTTCGAGTTTGAATAGTGGTCAGATTGAAGTTATCATGGATCGAAGACTCATGCAA
GATGATAATCGTGGCCTTGAGCAAGGTATCCAGGATAACAAGATTACAGCTAATCTATTT
CGAATACTACTAGAAAAAAGAAGTGCTGTTAATACGGAAGAAGAAAAGAAGTCGGTCAGT
TATCCTTCTCTCCTTAGCCACATAACTTCTTCTCTCATGAATCATCCAGTCATTCCAATG
GCAAATAAGTTCTCCTCACCTACCCTTGAGCTGCAAGGTGAATTCTCTCCATTACAGTCA
TCTTTGCCTTGTGACATTCATCTGGTTAATTTGAGAACAATACAGTCAAAGGTGGGCAAT
GGGCACTCCAATGAGGCAGCCTTGATCCTCCACAGAAAAGGGTTTGATTGTCGGTTCTCT
AGCAAAGGCACAGGGCTGTTTTGTTCTACTACTCAGGGAAAGATATTGGTACAGAAACTT
TTAAACAAGTTTATTGTCGAAAGTCTCACACCTTCATCACTATCCTTGATGCATTCACCT
CCCGGCACTCAGAATATAAGTGAGATCAACTTGAGTCCAATGGAAATCAGCACATTCCGA
ATCCAGTTGAGGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 1144 |
| Molecular Weight
| 131139.485 |
| Theoretical pI
| 7.573 |
| Pfam Domain Function
|
|
| Signals
|
Not Available
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Alpha-mannosidase 2
MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRNPRREGSFPQGQLSMLQEKIDHLER
LLAENNEIISNIRDSVINLSESVEDGPKSSQSNFSQGAGSHLLPSQLSLSVDTADCLFAS
QSGSHNSDVQMLDVYSLISFDNPDGGVWKQGFDITYESNEWDTEPLQVFVVPHSHNDPGW
LKTFNDYFRDKTQYIFNNMVLKLKEDSRRKFIWSEISYLSKWWDIIDIQKKDAVKSLIEN
GQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLENNIGVKPRSGWAIDPFGHSPTMAYL
LNRAGLSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDILCHMMPFYSYDIPHTC
GPDPKICCQFDFKRLPGGRFGCPWGVPPETIHPGNVQSRARMLLDQYRKKSKLFRTKVLL
APLGDDFRYCEYTEWDLQFKNYQQLFDYMNSQSKFKVKIQFGTLSDFFDALDKADETQRD
KGQSMFPVLSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESHLRAAEILYYFALRQAH
KYKINKFLSSSLYTALTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMVLEKIIG
NSAFLLILKDKLTYDSYSPDTFLEMDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDR
ISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKI
LESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMT
KEDGKHHEVNVQFSWYGTTIKRDKSGAYLFLPDGNAKPYVYTTPPFVRVTHGRIYSEVTC
FFDHVTHRVRLYHIQGIEGQSVEVSNIVDIRKVYNREIAMKISSDIKSQNRFYTDLNGYQ
IQPRMTLSKLPLQANVYPMTTMAYIQDAKHRLTLLSAQSLGVSSLNSGQIEVIMDRRLMQ
DDNRGLEQGIQDNKITANLFRILLEKRSAVNTEEEKKSVSYPSLLSHITSSLMNHPVIPM
ANKFSSPTLELQGEFSPLQSSLPCDIHLVNLRTIQSKVGNGHSNEAALILHRKGFDCRFS
SKGTGLFCSTTQGKILVQKLLNKFIVESLTPSSLSLMHSPPGTQNISEINLSPMEISTFR
IQLR
|
| External Links |
|---|
| GenBank ID Protein
| 51477714 |
| UniProtKB/Swiss-Prot ID
| Q16706 |
| UniProtKB/Swiss-Prot Entry Name
| MA2A1_HUMAN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| NM_002372.2 |
| GeneCard ID
| MAN2A1 |
| GenAtlas ID
| MAN2A1 |
| HGNC ID
| HGNC:6824 |
| References |
|---|
| General References
| - Liu T, Qian WJ, Gritsenko MA, Camp DG 2nd, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J Proteome Res. 2005 Nov-Dec;4(6):2070-80. [PubMed:16335952 ]
- Misago M, Liao YF, Kudo S, Eto S, Mattei MG, Moremen KW, Fukuda MN: Molecular cloning and expression of cDNAs encoding human alpha-mannosidase II and a previously unrecognized alpha-mannosidase IIx isozyme. Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11766-70. [PubMed:8524845 ]
|
